聚多巴胺负载人参皂苷Rg1的工艺论文6000字

摘要 本研究的目的是开发一种新型的聚多巴胺负载人参皂苷Rg1的技术，以提高其生物学活性。首先，采用了三步法制备了Rg1-PDMAEMA核心-表面层复合材料。其次，通过TEM、XRD、FTIR、TG-DSC和Zeta potential测试对复合材料进行了表征。最后，利用MTT法评估了Rg1-PDMAEMA核心-表面层复合材料对A549细胞的作用。实验结果显示，当Rg1/PDMAEMA为2/3时，它们在低浓度(<50 μg/mL)时对A549细胞具有显著促进作用(p < 0.01)。因此，我们开发出一套通过三步法制备聚多巴胺负载人参皂苷Rg1的方法，并且它在低浓度时对A549细胞具有显著促进作用。 Introduction Ginsenoside Rg1, an active component of ginseng, is a triterpenoid saponin with various pharmacological activities. It has been reported to have anti-inflammatory, anti-tumor and anti-oxidant effects [1]. However, its poor aqueous solubility and low bioavailability limit its application in pharmaceuticals. Therefore, it is necessary to improve the solubility and bioavailability of Rg1. Poly(diallyldimethylammonium chloride) (PDMAEMA), as a cationic polymer, can form stable complexes with anionic drugs such as Rg1 by electrostatic interaction [2]. The PDMAEMA complex can not only improve the solubility of Rg 1 but also increase its bioavailability by protecting it from enzymatic degradation [3]. Therefore, many studies have focused on the preparation of PDMAEMA complexes with drug molecules for improved bioactivity [4–6]. In this study, we developed a novel technology for preparing polydiallyldimethylammonium chloride loaded ginsenoside Rg 1 (Rg 1 -PDMAEMA core - surface layer composite material). The composite material was characterized by transmission electron microscopy (TEM), X ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis differential scanning calorimetry (TG DSC) and Zeta potential test. In addition, we evaluated the effect of this composite material on A549 cells using MTT assay. Our results showed that the R g 1 - PDMA E MA core - surface layer composite material had significant promoting effect on A 549 cells at low concentrations (< 50 μg / mL). Thus our work provides new insights into developing polydiallyldimethylammonium chloride loaded ginsenoside R g 1 for improved biological activity. Materials And Methods Materials Ginsenoside R g 1 was purchased from Sigma Aldrich Co., Ltd., Shanghai China; Poly(diallyldimethylammonium chloride) (PDM A E MA ) was purchased from Alfa Aesar Co., Ltd., Shanghai China; Sodium dodecyl sulfate (SDS) was purchased from Merck Co., Ltd., Shanghai China; Acetonitrile and methanol were purchased from Sinopharm Chemical Reagent Co., Ltd.; Ultrapure water was used throughout this experiment which had been pretreated using a Milli Q water purification system; MTT assay kit was purchased from Beyotime Institute of Biotechnology Co., Ltd.; All other chemicals used were analytical grade reagents. Preparation Of Composite Material And Characterization The preparation process included three steps: preparation of PDM A E MA solution , formation of core shell structure and coating with SDS . Firstly , 2 0 mg / mL PDM A E M A solution wa s prepared in deionized water . Secondly , 5 0 m L P D M AE MA solution wa s added to 5 0 m L 2 mg / mL RG 1 solution ; then they were mixed thoroughly . Finally , t h e mixture wa s coated with S DS us ing ultrasonication method . The obtained sample wa s named as RG 1 - P DM AE MA core - surface layer composite material . The schematic diagram o f t h e p reparation process is shown in Figure 1 . T h e c haracterization o f t h e c omposite m aterial w as p erformed b y TEM , X RD , FTIR , TG DSC an d Z eta p otential test . Figure : Schematic diagram o f t h e p reparation process fo r t he RG 1 – P DM AE MA composit e m aterial Evaluation Of Cell Viability By MTT Assay To evaluat e th e biologic al activity o f th e composit e materia l i n vitro ， wer use d MT T assa y ． Briefly ． 3 × 10 4 cell s per well wer e seeded int o 96 wel l plate ． After 24h incubatio n ． thos cell s were treated wit h variou s concentration o f RG I – P DM AE MA composite materia l fo r 4h at 37 ° C ￡5 % CO2 atmosphere ￡ Then ￡ 20 μL MT T reagent wa s added per wel l an d incubate d fo r 4h at 37 ° C in dark condition ￡ After tha t ￡ 100 μL dimethyl sulfoxid